University of Southern California

Analysis

Flow cytometry is a technology used to make fluorescence measurements of single cells moving through a laser-based sensing zone. On analysis-only instruments, cells pass through a closed system directly to waste and cannot be returned. Immunophenotyping, reporter gene assays, functional assays and cell cycle analyses are common applications performed on analysis cytometers.

The state-of-the-art instrumentation housed in the core facility is maintained by core personnel and covered by service contract support. All optimizations necessary to access proper instrument function are performed daily.

  1. The LSR II is available for reservation in half-hour increments. The CORES reservation system books the machine only. Additional charges are applied to compensate for core staff technical time whenever necessary. Available on the LSR II are the following items:
    • LSRII training, additional fees applied. (When ordering, choose “Assistant” to view available technician time.)
    • Expert operation, full service option, additional fees applied. (When ordering, choose “Assistant” to view available technician time.)
    • Analysis: Self operation, only trained personnel may use this service.
  2. The Cyan is available for reservation in half-hour increments. The CORES reservation system books the machine only. Additional charges are applied to compensate for core staff technical time when necessary. Available on the LSRII are the following items:
    • Cyan training, additional fees applied. (When ordering, choose “Assistant” to view available technician time.)
    • Expert operation, full service option, additional fees applied. (When ordering, choose “Assistant” to view available technician time.)
    • Analysis: Self operation, only trained personnel may use this service. (All end users seeking self operation must be trained by core personnel prior to booking appointments. Self operation privileges may be temporarily revoked by the core director if cytometer misuse or abuse becomes an issue. The end user will require additional training to regain access to core devices.)
  3. Interested in flow cytometry analysis? Initiate a discussion today by emailing Jeff Boyd at boydjeff@usc.edu and Bernadette Masinsin at masinsib@usc.edu.
  4. Additional theory provided here. (Link coming soon)
  5. Analysis FAQs