University of Southern California

Overview

The Flow Cytometry Core Facility provides the research community with state-of-the-art technology to selectively phenotype and isolate specific populations of cells. Using fluorescent probes and transgenic proteins, researchers monitor several aspects of their cell populations, including phenotype, cell viability, proliferation, cell cycle and cell activation. Additionally, cell purification by electrostatic cell sorting enables cutting-edge research on low frequency cells. This essential resource aides investigators performing research in cancer, cancer related areas, stem cell biology and regenerative medicine. With the increasing number of assays being developed that utilize flow cytometry, this resource supports a diverse group of investigators at USC and the Los Angeles region.

The facility has been used to address questions in adult and embryonic stem cell research, regenerative medicine, cellular immunology, cell biology, chemical carcinogenesis, radiology, human tumor cell cycle kinetics, mechanisms of drug action, and differences between patient samples and normal donors.

The facility houses a suite of digital high-speed multiparameter flow cytometers, and facility staff boast 50 years combined experience in this evolving field.

The facility is maintained by Lora Barsky (323-442-7942, lbarsky@usc.edu), an experienced director who provides instrument training, expert data analysis and consultation.

Events

Advance Data Analysis by Excyte
April 22, 2015
9 a.m.– 5 p.m.
Sue & Bill Gross CIRM Stem Cell Research Center; University of California, Irvine
expertcytometry.org

Compensation and Multiparameter Strategies
April 22, 2015
9 a.m.– 5 p.m.
Sue & Bill Gross CIRM Stem Cell Research Center; University of California, Irvine
flocyte.com

SoCal Flow Summit 2015
April 23–24, 2015
8 a.m.–8 p.m., 8 a.m.–5 p.m.
Becman Center; University of California, Irvine
socalflow.org

Interested in attending other educational seminars and other events? Please visit the USC Stem Cell events page.

Stream closeup (Photo by Tara Stanley)
LSR flow cell with lasers (Photo by Lora Barksy)
Flow cell (Photo by Tara Stanley)

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