Sorting: Aria #1

The BD SORP FACSAria IIu (ARIA #1) cell sorter is equipped with four lasers having 17 parameter excitation: 355nm (UV); 488nm (blue); 561nm (yellow-green); 633nm (red).

Up to 15 colors can be excited; configuration limitations exist.

Aria #1 uses sterile PBS as sheath buffer. Samples can be maintained at 4°–42° C (upon request; advance notice required). Aria #1 is contained inside a BioSero Class II Type B Biosafety Cabinet suitable for BSL 1- and 2-level work. Samples may be received in any of the following formats: 12x75mm (5mL)  FACS tubes or 15mL falcon-style centrifuge tubes.

Sorting nozzle sizes: 70um; 85um; 100um; 130um

Sample collection: 1- to 4-way for tubes (tube format limiting); 1 way to slides or dishes; 1 way for 6-, 12-, 24-, 48-, 96- and 384-well plates

Software: Diva 8.0.2 on Windows 7.  Data access available via USC network.

View ARIA #1 Configuration via FluoroFinder