Sorting: Aria #1
The BD SORP FACSAria I cell sorter is equipped with four lasers having 17 parameter excitation: 355nm (UV); 488nm (blue); 561nm (yellow); 633nm (red).
Up to 15 colors can be excited; configuration limitations exist.
Aria I uses sterile FACSFlow as sheath buffer. Samples can be maintained at 4°–42° C (upon request; advance notice required). Aria I is contained inside a BioSero Class II Type B Biosafety Cabinet suitable for BSL 1- and 2-level work. Samples may be received in any of the following formats: 1.7ml microcentrifuge tubes, 12x75mm (5mL) tubes or 15mL falcon-style centrifuge tubes.
Sorting nozzle sizes: 70um; 85um; 100um; 130um
Sample collection: 1- to 4-way for tubes (tube format limiting); 1 way to slides or dishes; 1 way for 6-, 12-, 24-, 48-, 96- and 384-well plates
Software: Diva 6.1.x on Windows XP. Networking is currently disabled.