Sorting: Aria #2

The BD FACSAria IIIu (ARIA #2) cell sorter is equipped with four lasers having 18 parameter excitation : 405nm (violet); 488nm (blue); 633nm (red); 561nm (yellow-green).

Up to 16 colors can be excited; configuration limitations exist.

Aria #2 uses sterile PBS as sheath buffer. Samples can be maintained at 4°–42° C (upon request; advance notice required). Aria #2 is contained inside a BioSero Class II Type B Biosafety Cabinet suitable for BSL 1- and 2-level work. Samples may be received in any of the following formats: 12x75mm (5mL) FACS tubes or 15mL falcon-style centrifuge tubes.

Sorting nozzle sizes: 70um; 85um; 100um; 130um

Sample collection: 1- to 4-way for tubes (tube format limiting); 1 way to slides or dishes; 1 way for 6-, 12-, 24-, 48-, 96- and 384-well plates

Software: Diva 8.0.2 on Windows 7. Data access available via USC network.

View ARIA #2 Configuration via FluoroFinder