University of Southern California

Sorting: Aria #2

The FACSAria II cell sorter is equipped with four lasers having 17 parameter excitation (pending summer 2014): 405nm (Violet); 488nm (Blue); 633nm (Red); 561nm (Yellow), pending.

Up to 15 colors can be excited; configuration limitations exist.

Aria II uses sterile FACSFlow as sheath buffer. Samples can be maintained at 4°–42° C (upon request; advance notice required). Aria II is contained inside a BioSero Class II Type B Biosafety Cabinet suitable for BSL 1- and 2-level work. Samples may be received in any of the following formats: 1.7ml micro-centrifuge tubes, 12x75mm (5mL) tubes or 15mL falcon-style centrifuge tubes.

Sorting nozzle sizes: 70um; 85um; 100um; 130um

Sample collection: 1- to 4-way for tubes (tube format limiting); 1 way to slides or dishes; 1 way for 6-, 12-, 24-, 48-, 96- and 384-well plates

Software: Diva 6.1.x on Windows XP. Diva 8 upgrade on Windows 7 is pending. Networking is currently disabled until computer upgrade is completed in summer 2014.

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Aria II crop