The BioRad S3e cell sorter is equipped with three lasers having 6 parameter excitation: 405nm (violet); 488nm (blue); 561nm (yellow-green). We have three different sets of filters for three instrument configurations.
Various 4 color panels can be detected; configuration limitations exist.
The S3e uses sterile sheath buffer. Samples can be maintained at 4°–42° C (upon request; advance notice required). Samples may only be received in 12x75mm (5mL) FACS tubes.
Sorting nozzle size: 100um
Sample collection: 1- or 2-way for tubes (12×75 FACS or 1.5mL eppendorf); 1 way to slides ; 1 way 96-well plate assembly (see staff for specialized 96-well plate).
Software: ProSort on Windows 10. Data access available via USC network.